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    15 June 2003, Volume 22 Issue 02
    The comparisons of cranial features between Chinese and African Holocene humans,and their implications
    LIU Wu; Emma Mbua; WU Xiu-jie; ; ZHANG Yin-yun
    2003, 22(02):  89-104. 
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    26 cranial features of Holocene African and Chinese skulls were observed and analyzed. Our results show that even though some feature seem to characterize African humans, the expression patterns of most cranial features used in present study resemble those of East Asian humans very much, which may suggest the similar trends of the characteristics of modern humans. We also found that more variations of cranial features occur in African human skulls than those of Chinese. The authors believe that these differences between African and Chinese indicate the physical characteristics of African hu- mans have wider variation ranges than those of East Asian populations. According to the high frequen- cies of some East Asian, European and Australian cranial features in African humans found in present study, the authors proposed some problems to be solved in future studies.
    First-time discovery of paleolithic remains in Zhejiang Province
    Zhang Sen-shui; Gao Xing; XU Xin-min
    2003, 22(02):  105-119. 
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    It is a preliminary report on the result of an archaeological reconnaissance conducted during October-November 2002, by a field team organized by the Institute of Vertebrate Paleontology and Paleoanthropology, Chinese Academy of Sciences in Beijing and the Cultural Relics and Archaeology Institute of Zhejiang Province in Hangzhou. One of the major achievements of the field survey is the discovery of Paleolithic artifacts for the first time in this coastal province, regarded by many as a signi- ficant breakthrough in archeological research in the region.
    A total of 333 pieces of lithic artifacts were collected from 31 sites during the survey (Fig. 1 and Table 1). These sites were located in two countiesin the north of the province, namely the Anji Coun- ty and the Changxing County. Some artifacts were unearthed in situ from a layer of reticulated red clay, a geological unit typical of the Middle Pleistocene deposit in South China, and a layer of red soilon top of it, while some were collected from brick factories, earth-taking grounds where their original places can be traced to the red clay levels. Based on preliminary examination of the artifacts, general features of these collected materials can be summarized as:
    1) The artifacts include cores, flakes, and retouched tools;
    2) Almost all the artifacts are large and heavy;
    3) Raw materialsinclude sandstone, quartzite, tuff, quart, flint, etc. Sandstone is the most frequently used material; Tool blanks are predominantly pebbles and cores ;In few cases, flakes were modified further into tools;
    4) Flakes were produced by direct hammer percussion ;No core platform preparation was detected;
    5) The category of tool includes chopper-chopping tools, picks, bifaces, spheroids, subsphe- roids, and scrapers. Chopper-chopping tool is the dominant type in the collection, followed by picks and subspheroids;
    6) Most of the tools were crudely trimmed by hard hammer percussion ;the majority of the retouch occurred on dorsal surface of the blanks.
    The characteristics of these artifacts discovered in North Zhejiang Province indicate that Paleolithic industries in that region are very similar to those found in the neighboring Anhui Province and Jiangsu Province. In general, they all demonstrate close affiliation to the Pebble-Tool Tradition in South China.
    Based on stratigraphy, artifacts discovered from the reticulated red clay in the these two counties belong to the Middle Pleistocene, and specimens collected from red clays above it is estimated as the Upper Pleistocene. Materials in surface collections exhibit no traces of long-distance movement, and therefore can be assigned to these two geological stages respectively based on stratigraphical sequences where they were exposed. Systematic examination on the accurate age of the archaeological remains at these sites will be followed.
    Zhejiang Province is very rich in Neolithic and historical archaeological remains and a very important place for studying the origin and development of early Chinese civilization in the Lower Yangtze River region. This discovery has profoundly prolonged the history of human adaptation in the area. It provides valuable data not only for the study of archaeological sequence in the region, but also for the study of the distribution and variability of the mainstream South China Paleolithic industry in general.
    A preliminary study on raw material exploitation in middle-upper paleolithic sites in Nihewan Basin
    DU Shui-sheng
    2003, 22(02):  121-130. 
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    In the past 20 years,Chinese scientists have discovered a large number of Paleolithic sites in the Nihewan Basin,North China,ranging from the Lower Paleolithic to the Upper Paleolithic. Among these sites,Xujiayao,Que' ergou and Banjingzi,situatd on the top of lake deposit or the third terrace, were dated to the Middle Paleolithic;the Lower Xinmiaozhuang, Shenquansi and Xibaimaying, found in the bottom of the second terrace,were dated to the early Upper Paleolithic;while Hutouliang, Jiqitan, Erheshanggou, the Upper Xinmiaozhuang, and Youfang were dated to the late Upper Paleolithic sites, and Toumafang and Yujiagou,situated in the upper section of the second terrace,were dated to Paleolithic-Neolithic transitional period. Stone artifacts collected from these sites provide basic materials for the study of the change of raw material exploitation strategies from the Middle to the Upper Paleolithic in the Nihewan Basin.
    Not all kinds of rock are suitable for stone tool manufacture, and the most frequently used materials by the Paleolithic people in the Nihewan Basin are vein quartz, chalcedony, volcano breccia, concealed crystal silicon, etc. Vein quartz is broadly distributed and can be easily obtained within the Nihewan Basin but with relatively low quality. Chalcedony and some volcanic rock are only located in limited areas,such as the Huiquanbao Village,Yangyuan Country and along some branches of the Sangganhe River. They can be easily obtained in these areas and have better quality than the vein quartz. Chalcedony materials are relatively hard with smooth and homogeneous internal consistency, but are generaly small in size,mostly smaller than 5 cm in diameter. Volcano breccia can be found in the cental areas of the basin,such as Guaijiawan and Xinmiaozhuagn. Concealed crystal silicon is distributed in the eastern areas of the basin,such as Youfang and Donggutuo. The latter two kinds of rock have very similar texture, that is,some part is fine while some is coarse.
    In order to compare raw material exploitation strategies between different ages and different areas, the basin where the target archaeological sites were located was divided into three parts: the East, Central, and the West.
    The East Part: Concealed crystal silicon is the principal raw material used in the eastern part of the Nihewan Basin. Though it is used as raw material by three sites—Banjingzi, Youfang and Toumafang, the exploitation degree is different from site to site. Among them, the Youfang site, the youngest in the sequence and nearest to the raw material source, yields well-made artifacts,which may be related to the application of microblade technology.
    The Central Part: Sites within the central part of the basin include Que'ergou, Daxiliangnangou, Hutouliang,Yujiagou and Xinmiaozhuang. Several kinds of raw material were exploited at these sites, but volcano breccia is the most frequently used material in this region.
    The West Part: Xujiayao, Shenquanis, Xibaimaying and Erheshenggou sites are situated in this district. While vein quartz and chalcedony are both used in the former three sites, the utilization of chalcedony here is unique. The exploitation of volcano breccia at the Erheshanggou site is another special character of this area.
    After comparing all the sites in the basin by their distance to the material sources, the preference of different raw materials selected, and the technology in processing these materials, some interesting phenomena were observed through time:
    The Middle Paleolithic: People at the three Middle Paleolithic sites, situated in different part in the basin, made use of different raw materials, i. e., lava and vein quartz as the main raw material at Que' ergou, vein quartz and chalcedony at Xujiayao, and concealed crystal silicon at Banjingzi. However, they are all local materials, obtained near the sites, within 10 km in distance.
    The early Upper Paleolithic: No distinct change in the way raw material were handled can be observed at sites of this period from the previous one. However, a piece of volcano breccia and some chalcedony pieces were found at Xinmiaozhuang,which might indicate that ancient people had extended their activity range to 10 kilometers by accident.
    The late Upper Paleolithic: In this period, great progress had been made in raw material selec- tion. First, vein quartz almost disappeared from the site because of its poor quality ;Chalcedony was seldom used, probably because its volume is too small to be used to produce microblade, even though its quality is fine. In the meantime, concealed crystal silicon and volcano breccia became the predominant raw material. Second, high-quality materials were transported beyound the ten-kilometer limitation. Volcano breccia,which is the characteristic material in the Central Part, appeared at the Youfang site of the East Part and the Erheshanggou site of the West Part,while the two sites are 70—80 km apart, and concealed crystal silicon was discovered in Yujiagou, 18 km west to the Youfang site in the East Part. Such material was also found at the Ma' anshan site. Third, some raw materials were flaked in their original place and only tool-blanks were brought to the site.
    Based on the above observations and analyses, it is concluded that:
    1. The activity territory of ancient people in the Nihewan Basin had been gradually extended from the Middle to Upper Paleolithic, from within 5 —10 km to more than 10 km, and the frequency of long- distance activity increased.
    2. More and more attentions were paid to the selection of higher-quality raw material through time.
    3. The use of poor-quality material, especially vein quartz, decreased through time, even though it is the most abundant material in the basin.
    4. Distance of raw material source to archaeological site played an important role in the way these materials were treated and processed. Materials that could be easilty obtained were usually exploited casually,while materials that could only be procured from long distance would be selected and processed carefully. Of course there are other factors that can influence the processing of certain raw materials. For instance, people occupied the Youfang site fabricated delicate tools on concealed crystal silicon, a material with its source very close to the site, as a result of the application of microblade technology.
    Stratigraphic anf paleoenvironmental studies at the Dadong Cave, Panxian
    WANG Wei; ; LIU Jun; HOU Ya-mei; Lynne A.Shepartz; Sari Miller-Antonio; William Jack Rink; SI Xin-qiang; HUANG Wei-wen
    2003, 22(02):  131-138. 
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    Deposits of the excavated area at the Dadong Cave in Panxian County, Guizhou Province, can be divided into three stratigraphic units, distributed in all parts of the main hall. Human fossils, stone artifacts and mammalian fauna were unearthed mainly from the second stratum, a breccia deposit. Angular rocks resulted from roof-fall and boulders are the main source of the deposits, filled with clay and sands,. The current U-series and ESR dating results indicate that early human activities in the cave began around 260 ka and ended at approximately 42 ka, which corresponds to Oxygen Isotope Stage 6 and 7. Strongly weathered dark deposits were detected from the lower breccia, suggesting a warm climatic period from 260 to 180 ka and corresponding to Oxygen Isotope Stage 7. The periodic climatic changes derived from the Dadong deposits can be compared with sequences of loess and glacia/ interglacial sequence of the Qinghai-Tibet Plateau.
    Paleoenvironmental and chronlogical analyses of the Daguling Paleolithic Locality, Xinyu Xity,Jiangxi Pronvince
    FANG Ying-san; LI Xu-sheng; YANG Da-yuang
    2003, 22(02):  139-144. 
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    The Daguling Paleolithic locality is situated at the second terrace of the Yuanshui River, a branch of the Ganjiang River in the Xinyu City, Jiangxi Province. More than 40 stone artifacts were collected from the site in 1997, which exhibit close affiliation with the Laterite Paleolithic Culture in South China. The representative deposit section at the site contains loess-like silty clay of more than 10 m thick. Through analyzing the granulometric, chemical composition and heavy mineral elements of the deposits and observing the surface characteristics of quartz grains by electron microscope, it is concluded that the deposits are aeolian, and the Xinyu loess was deposited in a climatic condition warmer and wetter than that of the loess of northern China and the Xiashu Loess in lower reaches of the Yangtze River. The OSL dating results indicate that the ages of the upper and middle part the deposits are 36. 1 ±2. 6 ka and 64. 2 ±4. 9 ka, respectively. Therefore, it is concluded that the Daguling cultural horizon is formed during the Upper Paleolithic.
    An analysis of congenitally anomaly of namber of permanent teeth in Beijing adolescents
    XU Mei-ru; HUO Yi-liang; L¨U Bing-feng
    2003, 22(02):  145-149. 
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    The purpose of this study is to investigate the incidence and mostly happened area of the congenitally missing teeth and supernumerary teeth in Beijing adolescent in order to approach the relation between the anomaly of tooth number and human evolution.
    Subjects and methods: 429 panoramic radiographs were observed and analyzed, 163 males and 266 females, aged 12 —23 years. None of permanent teeth have been extracted or hurted.
    Result: The incidence of congenitally missing permanent teeth (including third molar) is 42.19 %. The incidence of missing third molar is 35. 43 %. The mostly happenedmissing teeth are maxillary third molar, mandibular third molar, mandibular second premolar, mandibular median incisor, maxillary second premolar etc. the incidence of supernumerary teeth is 3.50 %, supernumerary teeth mostly happened are maxillary incisors.
    Conclusion: The incidence of missing teeth is higher than that of supernumerary teeth. Missing teeth mostly were found are the ones which have weaker function. The anomaly of tooth number is mostly due to hereditary factors. Congenitally tooth missing reflexes one of the evolutionary tendencies of the masticatory organ. The occurence of supernumerary teeth is a phenomenon of recapitalation.
    Study on physical anthropology of the Baiku Yao populations in China
    LUO Zai-gang; YANG Jia-li; YU Yue-sheng; RONG Ju-quan CEN Lin; REN Guang-xiang; LI Zhong-yan;
    2003, 22(02):  150-160. 
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    A somatological survey on 953 adults of Baiku Yao population, including 605 males and 348 females, living in Yaoshan Yao nationality Country of Libo County, Guizhou and Lihu, Baxu Yao nationality Country of Nandan County, Guangxi was carried out in October 1999 and May 2000. Their ages ranged from 22 to 55 years old. Their parents, grandparents and maternal grandparents were Baiku Yao nationality. Twenty eight items of observations and 63 measurements were made.
    The results of the survey are summarized briefly as follows:
    1. The physical characteristics of the Baiku Yao
    The Baiku Yao obviously belongsto the South Asian type of Mongoloid race. Most of the individuals belong to brachycephaly, hyperleptoproscopy, mesorrhiny. The stature of the Baiku Yao is of short type, the mean stature of males is 1556. 17 mm, and that of females is 1486.09mm.
    2. The relationship of the Baiku Yao and other nationalities
    On the basis of the dendrogram of the Baiku Yao and 16 groups of other minorities living in southern China, the physical character of the Baiku Yao is most closely related to the Maonan in Guizhou, Dong in Hunan, Miao in Yunnan, and is similar to those of the Miao, Yi, Dong and Yao in Guangxi. It is remotely related to the Miao, Shui in Guizhou, Tujia, Yao in Hunan and other nationalities.
    Disribution of Abo blood groups of the "Keriyan" in Xinjiang
    ZHANG Quan-chao; DUAN Ran-hui
    2003, 22(02):  161-162. 
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    The study of “Keriyan” living in the center of the Taklamakan desert of Xinjiang was carried out in 1958. Their ethnic attribution has not yet been decided. The distribution of ABO blood group systems was investigated on 93 individuals whose parents and grandparents are “Keriyan” too. The results showed that the gene frequencies for ABO blood group are r =0. 6095, p =0. 1886, q = 0. 2019. The χ2 test according to Hardy-Weinberg law is good. The gene frequecies of “Keriyan” for ABO blood group are concordant with theose of Uygur 、Kazak and Mongolian too.
    Contamination contorls and detection in ancient DNA studies
    YANG Dong-ya
    2003, 22(02):  163-173. 
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    With the advent of the PCR technique in molecular biology [20], DNA can now be extracted and analyzed from ancient remains [1, 51]. Ancient DNA studies hold great potential for anthropologists and archaeologists to address many important issues that cannot be well dealt with in conventional ways [5,9—14, 16—17]. However, the field is siill full of technical and interpretive challenges [28]. The most difflcult is proving that amplified DNA is authentic ancient DNA. The high risk of contamination is due to the fact that ancient DNA is highly degraded and only minute amounts are preserved,while the PCR technique is extremely sensitive and can easily pick up tiny amounts of contaminant DNA [7]. Contamination controls and detection therefore become extremely important in ancient DNA studies. This paper will discuss some practical guidelines that can be used to carry out effective contamination controls and detection in order to obtain authentic ancient DNA.
    Dedicated Laboratory for Ancient DNA Studies
    A dedicated laboratory is required for ancient DNA extraction and other pre-PCR work [28]. It is crucial to physically separate pre-PCR and post-PCR work [5]. The laboratory and all equipment should be dedicated to ancient DNA work. No modern DNA work should ever be carried out in the dedicated ancient DNA laboratory. Ideally, the pre-PCR laboratory should have UV-filtered ventilation system and positive pressure airflow. Sterile disposables and filtered tips should be used. Gloves, masks, boots and lab coats sliould be worn. 10 %bleach and UV light should be used to clean and irradiate the surfaces of benches and equipment to destroy contaminant DNA.
    Selection and Decontamination of Ancient Remain for DNA Studies.
    When selecting specimens for ancient DNA extraction, besides other criteria, the ease of decontaminating remains must be considered carefully since most remains excavated in the past had been contaminated by subsequent handling and analysis. There are several methods currently available for specimen decontamination [5, 30]. Physical methods remove the contaminated surface using sandpaper or electronic drills. Chemical decontamination uses chemicals such as 10 % bleach to damage and destroy surface contaminant DNA. Ultraviolet (UV) irradiation is another effective method for decontaminating specimens, reagents and other supplies [31]. UV can cause DNA to crosslink and preclude it from use in PCR amplification [7].
    DNA Extraction from Ancient Remains
    Selection of optimal DNA extraction methods and setup of blank extractions should be carried out in this step. Blank extraction should be used to monitor possible contamination of extraction reagents, commercial kits and the entire extraction process. Experiments that involve less steps or less human involvement should be considered advantageous.
    PCR Amplification of Ancient DNA
    The great difficulty in the amplification of ancient DNA is due to physical and chemical degradation of DNA templates [22, 51]. Ancient DNA can only be extracted in minute amounts, with small fragments and is often associated with PCR inhibitors, therefore, protocols for ancient DNA amplification must be optimized accordingly. Shorter target DNA fragments Should be sought since extracted DNA is usually less than 300 bp. The shorter the target fragment, the more templates will be potentially available for amplification. Obviously, with older remains, the difficulty to amplify longer fragments increases [22]. Thisfact can be used in the authentication of ancient DNA. Both negative and positive controls should be setup along with ancient DNA samples for PCR amplification [38]. Positive controls can be used to indicate whether PCR conditions are set up correctly and negative controls including blank extracts will show amplification products if contamination occurs. Multiple negative controls should be setup in order to more effectively monitor contamination [7]. For ancient human mtDNA,we have found that multiple quantified positive controls should also be used to indicate the sensitivity of individual PCR amplification and the level of contamination if it occurs [38].
    Quantification of Ancient DNA Templates
    Accurate estimation of the number of ancient DNA templates is of great assistance in determining whether amplified DNA is from authentic ancient DNA since greater numbers of ancient DNA templates result in a diminished likelihood of contamination [19, 43]. Competitive PCR can be used for the quantification of ancient DNA [39], but the estimated amount of templates may also include contaminant DNA. Another method has been proposed to examine the preservation state of ancient DNA through amino acid racemization analysis [42]. Although the analysis cannot produce any accurate estimation of ancient DNA templates, the preservation state can clearly indicate the possibility ofextracting DNA from very ancient remains such as fossils.
    Electrophoresis, Sequencing and cloning PCR Products and Sequence Analysis
    Once ancient DNA is amplified, it can be treated as any modem DNA sample would be and no special laboratory or equipment requirements are needed. Electrophoresis of multiple positive and negative controls should be used to quickly examine whether contamination occurs and the level of contamination if it occurs. Sequencing results can be of assistance in detecting contamination. For example, a DNA sample from one individual usually only contains one mtDNA sequence. A good indication of possible contamination is the presence of more than one type of mtDNA sequence or if the same type of mtDNA sequence is detected from many unrelated individuals.
    PCR products can be cloned to determine the number and percentage of different types of sequences present in PCR products. For more ancient remains such as fossils, cloning should be carried out since it is not only good for detecting contamination but also very useful in reconstructing an authentic ancient DNA sequence. When the number of DNA templates is extremely low and DNA itself is highly degraded, incorrect nucleotides may be incorporated into the synthesis of new DNA molecules and generate incorrect DNA sequences [43], or prematurely terminated DNA fragments may jump from one template to another and produce chimeric DNA sequences(jumping PCR) [44]. These amplification errors are generally random and can be detected though cloning and repeat experiments.
    Obtained ancient DNA sequences must make a phylogenetic sense [19] and or at very least should not contradict genetic rules and patterns. Otherwise, contamination should be suspected. For example, dinosaur DNA should be more similar to reptilian DNA than to mammalian DNA. In humans, one individual should only have two copies of nuclear DNA fragments. Special attention should also be given to the presence and detection of nuclear mtDNA amplification [45] and mtDNA' s heteroplasmy [46]. When these occur, they may complicate the detection of contaminant sequences.
    Reproducibility Test and Ancient DNA Authentication
    The reproducibility test is an integral part of ancient DNA research [43]. Replication of the entire ancient DNA processes should be undertaken to examine whether the same results can be obtained and is a requirement for the authentication of ancient DNA. Authentic ancient DNA and contaminant modern DNA have different “behavioral patterns” in the test. If DNA is authentic, the same DNA should be extracted, amplified and sequenced from different bones of the same individuals, in different laboratories and by different groups of researchers. Thus, it should be expected that different repeats generate the same DNA sequence. However, due to its random nature, contaminant DNA generally fails in reproducibility tests.
    The purpose of ancient DNA authentication is the analysis of all contamination controls, laboratory procedure' s and amplified DNA sequences to demonstrate that extracted and amplified DNA is authentic ancient DNA and not contaminant modern DNA [51]. Strict contamination controls are required but they cannot guarantee contamination-free results. There are also no absolute physical, chemical and biological criteria one can use to determine DNA' s antiquity. Thus, there is no way to directly authenticate ancient DNA based only on the DNA itself.
    Though we cannot directly determine whether amplified DNA is authentic or contaminant, logically, we can exclude one source and indirectly prove the other. Compared to the scarcity of ancient DNA templates, contaminant DNA is much more plentiful, making contamination with modern DNA an inevitable reality in ancient DNA studies. Therefore, one must analyze the possibility of contaminant DNA first before one can accept the result as authentic ancient DNA. Negative, positive controls and DNA sequence analyses are all capable of indicating contamination. Each individual control may not have a strong power in excluding contamination. When all controls and analyses do not indicate any contamination, statistically, there is likely no contamination.