Currently, there is no comprehensive study of the skin-fold thickness of Han nationality adults in various regions of China. From 2009 to 2013, we measured skin-fold thicknesses of face, biceps, triceps, subscapular, suprailiac, calfand calculated fat% of 23928 Han adults(13221 males, 13707 females) in Chinese twenty-twoprovinces. Using u-test to compare the city and the countryside inskin-fold thickness and analysing on correlation analysis of the six items of skin-fold thickness with latitude, longitude and age. The study found that on the whole, with the latitude reducing(from North to South), longitude increasing(from West to East) and age increasing, Han male skin-fold thickness increases and subcutaneous fat is thickened. With the increase of the latitude(from South to North) and the growth of the age, Han female skin-fold thickness increases, subcutaneous fat is thickened and PBF increases. The value of skin-fold thickness in urban Han was higher than that in rural Han. Han female skin-fold thickness values of subscapular and calf in urban are close to those in rural, the remaining four skin-fold thickness values in urban are greater than those in rural(P<0.01). Urban male PBF value is greater than the rural male, contrary to female. Among urban male, female and rural male, female, the skin-fold thickness of subscapular is the thickest, suprailiac is the second thickest, and the thinnest is biceps.