Abstract: This report used PCR-RFLP technique to screen 79 persons o f Han Nationality, 30 persons of Oroqen, 31 persons of Ewenki, 31 persons of Daur and 29 persons of Zhuang Nationality , all were randomly selected and healthy. Their MTHFR 677 site polymorphisms w ere detected and compared with each other.
Our results show ed that the polymorphic frequencies of this site are different among ethnic groups. Among 11 groups of Chinese and foreign populations, the V frequency o f Chinese Daur Nationality is highest, follow ed by that o f Han. This suggested that the frequencies of the variant V were higher in Asian groups.

Key words: Methylenetetrahydrofolate Reductase Gene, PCR-RFLP, Allele frequency